Impact of diesel oil effluent in the mucosal surface of the alimentary canal of Oreochromis nilotica (Linnaeus): a scanning electron microscopic study

Adverse effects of diesel oil on microanatomical structure of the alimentary canal of O. nilotica were studied using SEM observations. The study revealed irregular arrangement of the stratified epithelial cells along with fragmentation of the normal concentric pattem of microridges of the same cells in buccopharynx and oesophagus. The excessive secretion of mucus of buccopharynx and oesophagus were the salient changes caused by diesel oil pollution. The destruction and degeneration of the mucosal folds of stomach and intestine along with their epithelial cells exhibited a concrete hyperactivity resulting in abundant' secretion of mucus over the microridges of the epithelial cells. In the intestinal region the columnar epithelial cells showed tumefaction and microvilli of the plasma membrane of epithelial cells get heavily damaged. Disarray of the microridges of epithelial cells, excessive secretions of mucus formation of even cell sheet were the most conspicuous changes in rectum. It was concluded that chronic exposure of diesel oil may hamper the absorption of the nutrients through alimentary canal resulting into ill-growth and production of the fish.     

Large-Eddy Simulations of the Atmospheric Boundary Layer Using a New Subgrid-Scale Model – I. Slightly unstable and neutral cases

Subgrid-scale (SGS) modeling is a long-standing problem and a critical component in the large-eddy simulation (LES) of atmospheric boundary layer. A variety of SGS models with different levels of sophistication have been proposed for different needs, such as Smagorinsky's (1963) eddy viscosity model, Mason and Thomson's (1992) stochastic backscattering model, and Sullivan et al.'s (1994) near surface model. A modified Smagorinsky SGS model has been used in the LES version of Terminal Area Simulation System (TASS-LES). It has successfully simulated the buoyancy-dominated, convective atmospheric boundary layer flows, while simulations of the shear-dominated, slightly unstable, neutral, and stably stratified boundary layer flows are not so good. For the later, we used a simpler version of Sullivan et al.'s subgrid-scale model in which turbulent kinetic energy equation is not included and the model is still the first-order closure. A momentum profile matching approach is adopted in the proposed model. A series of simulations for shear-dominated, slightly unstable and neutral boundary layers are performed using different subgrid-scale models and different grid resolutions. The results are compared with those of Sullivan et al. (1994) and with empirical similarity relations for the surface layer. The simulations with the new SGS model appear to be far more satisfactory than those with the modified Smagorinsky model.     

Escape from competition: neighbors reduce Centaurea stoebe performance at home but not away

The greater abundance of some exotic plants in their nonnative ranges might be explained in part by biogeographic differences in the strength of competition, but these competitive effects have not been experimentally examined in the field. We compared the effects of neighbors on the growth and reproduction of spotted knapweed (Centaurea stoebe) in Europe, where it is native, and in Montana, where it is invasive. There were strong negative competitive effects of neighboring vegetation on C. stoebe growth and reproduction in Europe. In contrast, identical experiments in Montana resulted in insignificant impacts on C. stoebe. Although the mechanisms that produce this dramatic biogeographic difference in competitive outcome remain unknown, our results indicate that differences in net competitive interactions between ranges may contribute to the striking dominance of C. stoebe in parts of North America.     

Variations in seasonal phytoplankton assemblages as a response to environmental changes in the surface waters of a hypo saline coastal station along the Bhagirathi�CHooghly estuary

Due to the close proximity of the Bay of Bengal and the freshwater inflow of Bhagirathi-Hooghly, Diamond Harbour appeared as an important coastal station of the Bhagirathi-Hooghly estuary. The spatial and temporal composition and abundance of microphytoplankton species was examined in relation to physical and chemical surface water variables (i.e., salinity, nutrient, and temperature).The primary objective of the study was to observe the variations in phytoplankton species assemblages as a response to environmental variables. Hierarchical cluster analysis and non-metric multidimensional scaling were used to find out distinct seasonal groups based on the composition of phytoplankton. The results indicate that several key environmental factors like temperature, DIN content, and molar ratio of nutrients like DIN-DIP and DIN-DSi influenced seasonal phytoplankton assemblages within the estuary. The distribution of phytoplankton population showed two main groups where the blue-green and green algal populations favored the warmer conditions of summer and monsoon months, whereas the diatom population primarily flourished in the cooler months of autumn and winter.     

Active carbon-pools in rhizosphere of wheat (Triticum aestivum L.) grown under elevated atmospheric carbon dioxide concentration in a Typic Haplustept in sub-tropical India

Study on active and labile carbon-pools can serve as a clue for soil organic carbon dynamics on exposure to elevated level of CO2. Therefore, an experimental study was conducted in a Typic Haplustept in sub-tropical semi-arid India with wheat grown in open top chambers at ambient (370 micromol mol-1) and elevated (600 micromol mol-1) concentrations of atmospheric CO2. Elevated atmospheric CO2 caused increase in yield and carbon uptake by all plant parts, and their preferential partitioning to root. Increases in fresh root weight, volume and length have also been observed. Relative contribution of medium-sized root to total root length increased at the expense of very fine roots at elevated CO2 level. All active carbon-fractions gained due to elevated atmospheric CO2 concentration, and the order followed their relative labilities. All the C-pools have recorded a significant increase over initial status, and are expected to impart short-to-medium-term effect on soil carbon sequestration.     

Chromium phytoextraction from tannery effluent-contaminated soil by Crotalaria juncea infested with Pseudomonas fluorescens

The aim of present study was to remediate chromium (Cr)-contaminated soil by Crotalaria juncea in the presence of Pseudomonas fluorescens. Inoculation of P. fluorescens in pot soil grown with C. juncea significantly increased (~2-fold) the water-soluble (Ws) and exchangeable (Ex) Cr contents in contaminated soil under greenhouse condition. It also enhanced the chlorophyll content by 92 % and plant biomass by 99 % as compared to the uninoculated C. juncea plant. The analysis showed that root and shoot uptake of Cr in C. juncea inoculated by P. fluorescens was 3.08- and 2.82-fold, respectively. This research showed that the association of C. juncea and P. fluorescens could be a promising technology for increasing the soil Cr bioavailability and plant growth for successful phytoextraction of Cr from the contaminated soil.     

Analysis of the glyphosate herbicide in water,soil and food using derivatising agents

Glyphosate is used widely to control weeds. Glyphosate is a broad spectrum, non-selective, systemic and post-emergent herbicide. Glyphosate excessive use and impact on the environment is promoting the analysis of glyphosate in water, soil and food materials. Methods to analyse glyphosate at low levels are needed because glyphosate has a short half-life due to easy microbial degradation. Glyphosate has a high polarity and solubility in water, has high binding affinity with soil and is non-volatile. The absence of chromophoric groups in the molecular structure makes the detection difficult. Therefore, detection can be achieved by derivatisation, which makes glyphosate more volatile and stable for spectroscopic analysis. Derivatisation is commonly done by alkyl chloroformates, acylating agents, 9-fluoroenylmethylchloroformate, 4-methoxybenzenesulfonylfluoride and o-phthalaldehyde. Immunosensors allow detection at microlevels. Nanocrystals and nanotechnology allow detection at nanolevels. Here, we review methods to derivatise and analyse glyphosate.     

Intensive cattle grazing affects pasture litter-fall: an unrecognized nitrous oxide source

The rationale for this study came from observing grazing dairy cattle dropping freshly harvested plant material onto the soil surface, hereafter called litter-fall. The Intergovernmental Panel on Climate Change (IPCC) guidelines include NO emissions during pasture renewal but do not consider NO emissions that may result from litter-fall. The objectives of this study were to determine litter-fall rates and to assess indicative NO emission factors (EFs) for the dominant pasture species (perennial ryegrass [ L.] and white clover [ L.]). Herbage was vacuumed from intensively managed dairy pastures before and after 30 different grazing events when cows (84 cows ha) grazed for 24 h according to a rotational system; the interval between grazing events ranged from 21 to 30 d. A laboratory incubation study was performed to assess potential EF values for the pasture species at two soil moisture contents. Finely ground pasture material was incubated under controlled laboratory conditions with soil, and the NO emissions were measured until rates returned to control levels. On average, pre- and postgrazing dry matter yields per grazing event were 2516 ± 636 and 1167 ± 265 kg DM ha (±SD), respectively. Pregrazing litter was absent, whereas postgrazing fresh and senesced litter-fall rates were 53 ± 24 and 19 ± 18 kg DM ha, respectively. Annually, the rotational grazing system resulted in 12 grazing events where fresh litter-fall equaed to 16 kg N ha yr to the soil. Emission factors in the laboratory experiment indicated that the EF for perennial ryegrass and white clover ranged from 0.7 to 3.1%. If such EF values should also occur under field conditions, then we estimate that litter-fall induces an NO emission rate of 0.3 kg NO ha yr. Litter-fall as a source of NO in grazed pastures requires further assessment.     

Photocatalytic formation of hydrogen peroxide over highly porous illuminated ZnO and TiO2thin film

The photocatalytic formation of hydrogen peroxide over ZnO and TiO₂thin films has been investigated in aqueous phase in the presence of molecular oxygen as an electron acceptor. These films are highly porous and showed enhanced catalytic activity in the photochemical formation of hydrogen peroxide. The amount of H₂O₂formed during 2 hour light illumination is 4–6 μM and the rates of formation of hydrogen peroxide of both the films are almost comparable. The yield of hydrogen peroxide increases with the increase in irradiation time and a trend of steady state concentration of H₂O₂is observed in the case of TiO₂thin film. Photodissolution of ZnO particles is observed in some extent during the process of prolonged UV light illumination.     

Chromium toxicity and tolerance in plants

Chromium (Cr) is the second most common metal contaminant in ground water, soil, and sediments due to its wide industrial application, hence posing a serious environmental concern. Among various valence states, Cr(III) and Cr(VI) are the most stable forms. Cr(VI) is the most persistent in the soil and is highly toxic for biota. Since Cr is a non-essential element for plants, there is no uptake mechanism; Cr is taken up along essential elements such as sulfate through sulfate transporters. Cr accumulation in plants causes high toxicity in terms of reduction in growth and biomass accumulation, and Cr induces structural alterations. Cr interferes with photosynthetic and respiration processes, and water and minerals uptake mechanism. Various enzymatic activities related to starch and nitrogen metabolism are decreased by Cr toxicity either by direct interference with the enzymes or through the production of reactive oxygen species. Cr causes oxidative damage by destruction of membrane lipids and DNA damage. Cr may even cause the death of plant species. Few plant species are able to accumulate high amount of Cr without being damaged. Such Cr-tolerant, hyperaccumulator plants are exploited for their bioremediation property. The present review discusses Cr availability in the environment, Cr transfer to biota, toxicity issues, effect on germination and plant growth, morphological and ultrastructural aberrations, biochemical and physiological alterations, effect on metabolic processes, Cr-induced alterations at the molecular level, Cr hyperaccumulation and Cr detoxification mechanism, and the role of arbuscular mycorrhizae in Cr toxicity, in plants.